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                        E. WI DD E. N T         System Microscope

OLYM PUS         BX53/BX43/BX46

                             BX3 Series

The New Standard in Ergonomics and Productivity
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                  Your Choice
                  for Clinical Applications

                  BX3 series combines ergonomics with Olympus' optical
                  technology in three models— the BX53, BX43, and Bx46
                  microscopes. BX3 series microscopes have an ergonomic
                  design that helos keep users comfortable during extended
                  periods of use and an intuitive control layout for fast, efficient
                  observation and imaging.
                  Designed for laboratory and clinical applications, white LED
                  illumination has a high luminosity and color-rendering index so
                  users can see their samples in true-to-life colors.

                  HeLa Cells (FISH Stain)      Cervical Cells (Papanicolaou Stain)

Rat Kidney (PAM)  Blood Sample (Giemsa Stain)  EML4-ALK Fused-gene Lung (Immunostaining)
BXOS

For Teaching and Challenging Applications

With an LED illuminator equivalent to or better than a 100 W halogen lamp, the BX53
microscope delivers brightness that's appropriate for teaching and various contrast
methods. Customize your microscope with modular units based on the observation
methods you want to use. Choose from options including condensers, nosepieces, a
rotating stage, objectives, and intermediate optics optimized for various observation
methods, including phase contrast and fluorescence.

Acquire Precise Images with X Line Objectives                       Bright LED Lighting Designed for Pathology and Laboratory
                                                                    Designed with spectral characteristics that mimic halogen light
Improved flatness, numerical aperture, and chromatic aberration     sources, the BX3 series' LED illumination enables users to clearly
                                                                    view the purple, cyan, and pink colors important in pathology, but
combine to deliver clear, high-resolution images with excellent     typically difficult to see using LEDs. Users get the benefits of an
                                                                    LED, including consistent color temperatures and long use life,
color reproduction. The objectives’ superior chromatic aberration   without the typical trade offs.
management delivers better color accuracy across the entire
spectrum. The elimination of violet color aberration creates clear
whites and vivid pinks, improving contrast and sharpness.

                                                                                               === Halogen Lamp           === Commercially Available
                                                                                                      + Day Light Filter        White LED

                                                                              T    T      T            T      T                * This graph shows the spectral

                                                                                   Spectral Characteristics*                   characteristics of each light source

                                                                                                                               regularized with the luminosity curve.

                                                                                                                               It does not compare the strength of

                                                                                                                               light for each light source.

                                                                                      Mn

                                                                    380  430  480T 530    580  630     T  680 T  730      780

                                                                                      Wavelength [nm]
Bright Images in Multi-Head Configurations                        Coded units to integrate with imaging software
Multi-head discussion systems are essential for training and      Add an optional coded nosepiece to your BX53 microscope to
education. With the BX53 microscope's LED illumination, up to 26  automatically record and share magnification setting information
participants can view clear, bright images.                       for post-imaging treatments. The metadata is automatically sent to

26 heads are only for brightfield observation.                    cellSens software, helping minimize mistakes and scaling errors.

                                                                  cellSens is not for clinical diagnostic use.

                                                                      Magnification    Sent to cellSens
                                                                  Setting Information

                                                                      Image Data
Excellent Performance in a Cost-Effective System

BxX43 microscopes are modular, offering the versatility to change between cost-efficient
and advanced configurations depending on your needs. Choose from a wide variety of
modular components, including ergonomic observation tubes and stages, to customize

your microscope to your application.

White LED with High Color Rendering—                            Maintain Brightness when Changing Magnifications
Equivalent to a 30 W Halogen Lamp                               The BX3 series’ light intensity manager eliminates the step of
The Bx43 microscope utilizes a high color rendering white LED   adjusting lamp brightness when changing magnification. By
with a luminosity equivalent to a 30 W halogen lamp. The long-  maintaining uniform brightness at any magnification, users can
lasting LED provides a consistent color temperature at any      achieve their observations quickly and with reduced eye strain.
brightness level.

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                                                                                        Cervical Cell (Papanicolaou Stain)
Adjust the Scope to Fit Your Posture
Our most ergonomic option moves up and down, tilts, and extends forward and
back so you can move it closer to you. With this one component, users of nearly
any height can adjust the scope so that they're comfortable. The flexible
ergonomic tube is suitable for labs where multiple users share a microscope
since each can adjust it to accommodate their height and posture.

 ©                      ©               ©

Tilts: 0 to 27 degrees  Extends: 55 mm  Lifts: 45 mm
Various Observation Methods

Brightfield

Get Bright Images with Excellent Resolution/Flatness at All Magnifications
Olympus' diverse line of condensers enables users to choose what they need for their application. For example, the U-SC3 swing-out
condenser is suitable for observations from 1.25X to 100X, the U-LC is optimized for consecutive observations from 2X to 100X (dry).

                                                          A

                                                                                                                                         Lung Tissue with EML4-ALK fusion gene (Haemotoxylin and Eosin Stain)

Fluorescence

Bright Fluorescence Imaging
Users can choose from a universal reflected illuminator or a coded fluorescence illuminator. Eight fluorescence mirror units can be attached to
the microscope for efficient multi-color fluorescence observations. High performance filters provide efficient and bright fluorescence images.

Muscle Tissue (Fluorescence)  Mammary Gland Tissue (Fluorescence)
Phase Contrast

High-Contrast, High-Resolution Imaging
High-contrast phase imaging enables close observation of the interior of a cell and live bacteria. Use the UPLFLN-PH or PLN-PH objectives
for phase contrast observation from 10X to 100X. With the U-PCD2 phase/darkfield condenser, users can view specimens in brightfield or
darkfield. Simultaneous observation with reflected light fluorescence microscopy is also possible.

Endothelial Cells                                                             NRK-52E Cells

Darkfield                                                                     Polarized Light

Excellent Darkfield Effect from Low to High Magnification                     High-Resolution View of Double Refraction Structure in Cells
Choose from a 10X to 100X dry darkfield condenser or a 20X to                 Various compensators make it possible to observe a wide range of
100X oil immersion darkfield condenser.                                       retardation.

DAB-stained brain section, 30 um thick, 20x UPLSAPO Darkfield, DAB specimen   Uric Acid Crystals
showing anterograde and retrograde transport of Cholera Toxin B tracer in a
chick brain with an injection of tracer in the medial Arcopallium (amygdala)

                                                                                                                                                10
EvidentScientific.com                                                  Images are courtesy of:

E VIDENT' } EVIDENT CORPORATION                                                       Noriko Motoi, M.D., Ph.D. and Yuichi Ishikawa, M.D., Ph.D.

OLYMPUS                                                                      Division of Pathology, The Cancer Institute,
                                                                             JAPANESE FOUNDATION FOR CANCER RESEARCH
                                                                             (P.9 top right)
                                           ¢ EVIDENT CORPORATION is ISO14001 certified.
                                           * EVIDENT CORPORATION is ISO9001 certified.
                                           ¢ EVIDENT CORPORATION is IS013485 certified.

                                             ¢ IIlumination devices for microscope have suggested lifetimes.
                                                Periodic inspections are required. Please visit our website for details.

                                                 * All company and product names are registered trademarks and/or trademarks of their respective owners.

                                          ** SIpmeacgiefsicaotniotnhse aPnCd maopnpiteoarrsancarees siamreulastuebdj.ect to change without any notice or obligation on the part of the manufacturer.

                                 so mm

                                                                                                                     Printed in Japan M1696E-042022